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KMID : 0613820100200040614
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Journal of Life Science
2010 Volume.20 No. 4 p.614 ~ p.617
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Genetic Differences between Wild and Cultured Populations in Olive Flounder in Korea Based on Mitochondrial DNA Analysis
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Kim Mi-Jung
Kim Kyung-Kil
Park Jung-Youn
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Abstract
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We sequenced a 522 bp fragment including the tRNAThr, tRNAPro gene and the first half of the control region from 29 wild and cultured olive flounder specimens from Korea. Out of 522 nucleotide sites, 49 (9.4%) were variable, 23 haplotypes being found. Most haplotypes are unique in the wild population and only four were shared by cultured specimins. The nucleotide diversity and differences between wild and cultured populations were 0.025±0.013 and 0.015±0.008, and 12.94±6.00 and 7.83±3.75, respectively. Haplotype diversity was 0.98±0.02 and 0.49±0.09 in the wild and cultured populations, respectively. These results show that marked reductions of genetic variability in the hatchery strains were observed in the number of mitochondrial DNA haplotypes and haplotype diversity when compared to the wild populations. Furthermore, we detected significant population differentiation between both populations. The mtDNA sequencing technique used to evaluate the genetic variability of hatchery strains compared to that of the wild population is potential for genetic monitoring of olive flounder hatchery stocks.
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KEYWORD
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Olive flounder, mitochondrial DNA, diversity, differentiation
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